Development of EST-SSR Markers forPrimula ovalifolia(Primulaceae) by Transcriptome Sequencing
نویسندگان
چکیده
منابع مشابه
Development of EST-SSR and TRAP markers from transcriptome sequencing data of the mango.
Mango is one of the most commercially important fruit crops in tropical and subtropical regions. To increase the efficiency of breeding strategies, two EST-derived marker systems were developed in the present study using information from the mango fruit transcriptome. Using simple sequence repeats, 218 of 230 primer pairs showed stable amplification for 7 mango genotypes with amplicons ranging ...
متن کاملDevelopment and characterization of EST-SSR markers via transcriptome sequencing in Brainea insignis (Aspleniaceae s.l.)1
Premise of the study Brainea insignis (Aspleniaceae) is an endangered tree fern in China whose wild populations have been seriously damaged due to overexploitation. Expressed sequence tag-simple sequence repeat (EST-SSR) primers were developed to investigate its genetic diversity and provide resources for future conservation studies. Methods and Results We obtained 72,897 unigenes of B. insig...
متن کاملDevelopment of EST-SSR markers for Primula ovalifolia (Primulaceae) by transcriptome sequencing1
Premise of the study Microsatellite primers were developed for Primula ovalifolia, a member of Primula section Petiolares (Primulaceae), to study the population genetics and species delimitation in this section. Methods and Results A total of 4753 markers were successfully designated from 5139 putative simple sequence repeat loci. We isolated 38 expressed sequence tag-simple sequence repeat m...
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Caragana korshinskii Kom. is widely distributed in various habitats, including gravel desert, clay desert, fixed and semi-fixed sand, and saline land in the Asian and African deserts. To date, no previous genomic information or EST-SSR marker has been reported in Caragana Fabr. genus. In this study, more than two billion bases of high-quality sequence of C. korshinskii were generated by using i...
متن کاملTranscriptome Sequencing of Mung Bean (Vigna radiate L.) Genes and the Identification of EST-SSR Markers
Mung bean (Vigna radiate (L.) Wilczek) is an important traditional food legume crop, with high economic and nutritional value. It is widely grown in China and other Asian countries. Despite its importance, genomic information is currently unavailable for this crop plant species or some of its close relatives in the Vigna genus. In this study, more than 103 million high quality cDNA sequence rea...
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ژورنال
عنوان ژورنال: Applications in Plant Sciences
سال: 2017
ISSN: 2168-0450
DOI: 10.3732/apps.1700100